Lab Test Report of Pure Ghee: How to Read FSSAI & NABL Results

Published on January 17, 2026 11 min read buying guide โ€ข lab testing โ€ข fssai โ€ข nabl โ€ข purity

You ordered expensive A2 ghee, received a fancy jar with 'pure' written everywhere, but have zero way to verify if it actually is pure. A lab test report is the only scientific proof of ghee purity, yet 95% of buyers have never read one because nobody explains what those numbers actually mean.

This guide teaches you to read ghee lab reports like an expert, understand every parameter from fat percentage to Bauduoin Test, and spot fake certificates. First, understand how to identify pure ghee.

๐Ÿ“Š Key Ghee Purity Standards

99%+
Minimum Fat Content
<0.5%
Maximum Moisture
Negative
Bauduoin Test
28-35
RM Value (Cow)

Why Ghee Lab Testing Matters for Every Buyer

The ghee market in India is plagued with adulteration. FSSAI surveys repeatedly show that 30-40% of ghee samples fail purity tests. The most common adulterants are refined vegetable oils, vanaspati, and animal body fats that are invisible to taste and smell but show up clearly in laboratory analysis. Without lab verification, you are trusting marketing over science.

Lab testing is the only way to verify claims like pure ghee or A2 ghee. A lab-tested ghee brand that shares reports openly demonstrates confidence in quality. Brands that avoid sharing lab data may have something to hide. Understanding how to read these reports puts the power of verification in your hands.

FSSAI vs NABL: What Each Certification Means

What is FSSAI and Why It Matters

FSSAI (Food Safety and Standards Authority of India) is the regulatory body that sets legal standards for all food products sold in India, including ghee. Every ghee manufacturer must have an FSSAI license to legally sell their product. The FSSAI sets minimum quality parameters that ghee must meet, including fat content, moisture levels, and absence of adulterants.

When you see an FSSAI license number on ghee packaging, it means the manufacturer is registered with the food safety authority. However, this does not guarantee that every batch meets standards. It only means the business is legally authorized to produce ghee. You can verify any FSSAI license at foscos.fssai.gov.in by entering the license number.

What is NABL and Why Lab Accreditation Matters

NABL (National Accreditation Board for Testing and Calibration Laboratories) accredits laboratories that meet international testing standards (ISO/IEC 17025). When a ghee sample is tested at a NABL accredited lab, you can trust that the testing methods, equipment calibration, and quality controls meet global standards.

Non-NABL labs may use outdated methods, poorly maintained equipment, or lack proper quality controls. Some may even produce reports favoring clients who pay them. Always verify NABL accreditation at nabl-india.org before trusting any lab report. The accreditation number and validity period should be clearly printed on the test certificate.

Key Difference

FSSAI = The rules (what ghee must contain to be legal)
NABL = The referee (labs qualified to check if those rules are followed)

Critical Parameters in a Ghee Lab Test Report

A comprehensive ghee lab test report contains multiple parameters. Understanding each one helps you evaluate purity accurately. Here are the most important parameters and what values to look for.

Milk Fat Content (Minimum 99%)

This is the most fundamental parameter. FSSAI mandates that pure ghee must contain minimum 99% milk fat. Premium quality ghee often shows 99.5% or higher. The remaining fraction (up to 1%) comprises naturally occurring moisture and trace milk solids that remain after clarification.

If a lab report shows fat content below 99%, the ghee is either adulterated with cheaper fats or was manufactured with poor processes. Some adulterators mix refined vegetable oils (which are much cheaper) and still achieve 95-98% fat content on tests. This is why fat percentage alone is not enough. You need additional tests like Bauduoin.

Bauduoin Test (Must Be Negative)

The Bauduoin Test is the most important adulteration detection test for ghee. It specifically detects vegetable oil presence, particularly sesame oil which is commonly used to adulterate ghee. The result must show Negative for pure ghee.

A positive Bauduoin Test means the ghee contains vegetable oil and should be rejected immediately. This test is so critical that FSSAI mandates it for all ghee samples. Home tests cannot detect vegetable oil mixing at low levels, but the Bauduoin Test catches even small percentages. This is why brands to avoid often fail this specific test.

Moisture Content (Maximum 0.5%)

Moisture in ghee should be below 0.5% according to FSSAI. High moisture indicates incomplete clarification during manufacturing. Excess moisture reduces shelf life, promotes microbial growth, and affects texture and taste.

Traditional Bilona ghee made with proper slow heating typically shows lower moisture (0.1-0.3%) compared to industrial speed processing. Lower moisture also means better preservation of nutrients and longer freshness.

Reichert-Meissl (RM) Value

The RM Value measures volatile fatty acids in ghee and helps confirm that the fat is genuine milk fat. For cow ghee, the RM Value should typically be 28-35. Buffalo ghee shows slightly higher RM values (32-38).

This test is important because adulterators sometimes use animal body fat (tallow) instead of vegetable oils. While body fat may pass the Bauduoin Test, it shows abnormal RM Values. Combined with other parameters, the RM Value helps identify genuine milk fat origin.

Free Fatty Acid (FFA) Level

FFA indicates freshness and storage quality. Fresh, properly stored ghee should have FFA below 3%. Higher FFA levels suggest the ghee is old, was stored improperly, or was made from poor quality cream or butter.

As ghee ages or is exposed to heat and light, fats break down and FFA increases. This affects taste (becoming rancid) and nutritional quality. FFA testing helps identify ghee that may have been sitting in warehouses for months before reaching you. Learn more about ghee storage and shelf life.

Peroxide Value

Peroxide Value measures oxidation level in ghee. Fresh ghee should have low peroxide values. As ghee oxidizes (due to heat, light, or oxygen exposure), peroxide value increases. High peroxide indicates rancidity and degraded quality.

This parameter is especially important if you buy ghee in bulk or store it for extended periods. Ghee with high peroxide value may look and smell acceptable but has already begun degrading nutritionally.

Quick Reference: Ghee Test Parameters

Parameter Pass Value Fail Indicates
Milk Fat Content โ‰ฅ99% Adulteration or poor processing
Bauduoin Test Negative Vegetable oil adulteration
Moisture <0.5% Incomplete clarification
RM Value (Cow) 28-35 Non-milk fat or wrong animal
Free Fatty Acid <3% Old or improperly stored
Peroxide Value Low Oxidation and rancidity

How to Verify If a Ghee Lab Report is Genuine

Unfortunately, fake lab reports exist. Some unscrupulous sellers create forged certificates or use reports from labs that produce favorable results for a fee. Here is how to verify authenticity before trusting any ghee lab report.

Lab Report Verification Checklist

  • Check NABL accreditation: Verify the lab's NABL number at nabl-india.org. Confirm it is valid and not expired.
  • Verify unique report number: Every genuine report has a unique reference number. Call the lab to confirm this specific report exists in their records.
  • Check testing date: Reports should be recent (within 2-3 months for batch-wise testing). Old reports may not reflect current product quality.
  • Confirm lab exists: Search the laboratory name and verify their address, contact number, and FSSAI license independently.
  • Look for test method references: Genuine reports cite specific test methods (like IS 3508 for ghee testing). Generic reports without method references are suspicious.
  • Verify signatures: Reports should have authorized signatory details with name and designation. Stamps alone are easily forged.

Brands that maintain genuine quality testing welcome verification calls. If a brand discourages you from verifying their lab reports or cannot provide recent batch-specific reports, treat this as a warning sign. Transparent brands like Authentic Urban go beyond lab reports by providing video proof of each order being prepared.

A2 Ghee: Can Lab Tests Verify A2 Claims?

This is an important question many buyers ask. Standard FSSAI lab tests cannot differentiate between A2 and A1 milk fat. The A2 vs A1 difference is about beta-casein protein structure in milk, and during ghee preparation, proteins are largely removed. What remains is mostly pure fat.

To verify A2 claims, you need specialized protein testing on the source milk, not the ghee itself. This is expensive and rarely done commercially. For A2 ghee verification, you must rely on breed documentation and source traceability rather than standard lab tests. Look for brands that show their cows (indigenous breeds like Gir, Sahiwal, Red Sindhi) and milk sourcing process rather than just claiming A2 on labels.

Standard ghee lab tests will tell you if the ghee is pure milk fat without adulteration. They do not tell you which cow breed the milk came from. This is why sourcing transparency and video verification matter as much as lab reports for A2 ghee buyers.

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Common Myths About Ghee Lab Testing

โŒ Myth: "If the label says pure ghee, lab testing is unnecessary"

Reality: FSSAI surveys consistently show that 30-40% of ghee samples fail purity tests despite labels claiming purity. Labels are marketing, not verification. The Bauduoin Test, RM Value, and fat percentage can only be accurately measured in a laboratory. Home tests like palm melting or water drop tests can detect crude adulteration but miss sophisticated mixing of refined oils. For families concerned about genuine purity, always ask brands for NABL certified lab reports.

โŒ Myth: "Any lab can test ghee accurately so the certificate always matters"

Reality: Not all labs are equal. Only NABL accredited laboratories follow internationally validated testing methods (ISO 17025). Non-accredited labs may use outdated equipment, improper techniques, or lack quality controls, producing unreliable results. Some labs may even be compromised to produce favorable reports for paying clients. Always verify NABL accreditation on nabl-india.org before trusting a ghee lab report. The accreditation number should be clearly printed on the test certificate.

โŒ Myth: "Higher price means the ghee has already been lab tested"

Reality: Price and testing are not correlated. Many expensive ghee brands never publish lab reports, while some mid-range brands maintain strict quality testing. Premium pricing often covers marketing, packaging, and brand positioning rather than quality verification costs. The only way to know if a brand tests their ghee is to directly ask for lab reports. Transparent brands provide this without hesitation. If a brand refuses or deflects, their claims remain unverified regardless of price point.

โŒ Myth: "Home purity tests like palm melting or granulation are as reliable as lab tests"

Reality: Home tests can only detect very crude adulteration. A skilled adulterator can mix refined vegetable oil in proportions that pass all home tests while still compromising purity. The Bauduoin Test (for vegetable oil), Reichert-Meissl Value (for milk fat authenticity), and precise fat percentage measurement require laboratory-grade equipment and trained technicians. Home tests are useful first-level filters, but they cannot replace NABL lab verification for serious purity assurance.

Questions to Ask Ghee Brands About Lab Testing

When evaluating a ghee brand, asking the right questions reveals their commitment to quality. Here are specific questions that separate truly transparent brands from those hiding behind marketing.

1. Can you share your most recent NABL lab test report?

Good brands share reports immediately. Hesitation or excuses indicate problems.

2. How frequently do you test your ghee batches?

Batch-wise or monthly testing shows commitment. Annual or no testing is inadequate.

3. Which NABL accredited lab do you use for testing?

Known labs with verifiable NABL accreditation add credibility.

4. Can I verify the lab report directly with the testing laboratory?

Genuine reports can be verified. Fake reports cannot withstand independent verification.

5. What was the Bauduoin Test result in your last report?

This specific question tests if the brand actually understands quality parameters.

Choosing ghee based on these answers rather than packaging claims leads to better purchases. Brands that pass this questioning demonstrate genuine quality focus.

Beyond Lab Reports: See Your Ghee Being Made

Lab reports verify purity. Video proof verifies process. With Authentic Urban, you see YOUR specific jar being prepared using traditional Bilona method, from curd to ghee to packaging.

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Frequently Asked Questions About Ghee Lab Testing

What are the key parameters to check in a ghee lab test report?

The most critical parameters in a ghee lab test report are: (1) Milk Fat content which must be minimum 99% for pure ghee, (2) Moisture content which should be below 0.5%, (3) Free Fatty Acid (FFA) level which indicates freshness and should be under 3%, (4) Bauduoin Test (BT) which must show negative to confirm absence of vegetable oil adulteration, (5) Peroxide Value which measures oxidation and should be low for fresh ghee, and (6) Reichert-Meissl (RM) Value which identifies genuine milk fat origin, typically 28-35 for cow ghee. Always ensure the lab is NABL accredited for reliable results.

What is the difference between FSSAI and NABL certification for ghee testing?

FSSAI (Food Safety and Standards Authority of India) sets the legal standards that ghee must meet for sale in India. It defines minimum requirements for fat content, moisture, and purity parameters. NABL (National Accreditation Board for Testing and Calibration Laboratories) accredits laboratories that perform testing. When a lab is NABL accredited, it means their testing methods, equipment, and processes meet international ISO standards. For reliable ghee quality verification, get your ghee tested at a NABL accredited lab that follows FSSAI testing protocols. The combination ensures both accurate testing and legal compliance.

How can I verify if a ghee brand lab report is genuine and not fake?

To verify authenticity of a ghee lab report: (1) Check for NABL accreditation number on the report and verify it on the NABL website nabl-india.org, (2) Look for unique report number, testing date, and sample reference details, (3) Verify the testing laboratory exists and is operational by searching their FSSAI license, (4) Cross-check the lab address and contact details independently, (5) Ensure the report mentions specific test methods used (like IS 3508 for ghee), (6) Look for authorized signatures and official stamps. Brands that provide transparent lab reports usually also offer video proof of production like Authentic Urban does.

What does negative Bauduoin Test mean in a ghee lab report?

A negative Bauduoin Test is excellent news. This test specifically detects the presence of sesame oil and other vegetable oils in ghee. When the result shows negative, it confirms that the ghee sample contains no detectable vegetable oil adulteration. This is one of the most important purity indicators because vegetable oil mixing is the most common form of ghee fraud in India. If the Bauduoin Test shows positive, the ghee is adulterated and should not be purchased. FSSAI mandates this test for every ghee sample because vegetable oil adulteration is widespread and difficult to detect by taste or smell alone.

What should be the milk fat percentage in pure ghee according to FSSAI?

According to FSSAI standards, pure ghee must contain minimum 99% milk fat. Premium quality ghee often shows 99.5% or higher milk fat content. The remaining fraction (up to 1%) comprises naturally occurring moisture and trace milk solids. If a lab report shows milk fat below 99%, it indicates either adulteration with cheaper fats or poor manufacturing processes. Note that the label "pure ghee" or "100% ghee" on packaging does not guarantee actual purity. Only a lab test can verify true milk fat percentage. This is why transparent brands share batch-wise lab reports with customers.

How often should ghee brands test their products in NABL labs?

For optimal quality assurance, ghee brands should conduct NABL lab testing at least once per batch or once per month for continuous production. Large-scale manufacturers following good practices test every production batch before release. Some parameters like moisture and fat content can be tested in-house daily, but comprehensive purity testing including adulteration detection requires NABL accredited lab analysis. Brands that claim transparency but cannot show recent batch-specific lab reports (within 2-3 months) may not be maintaining adequate quality control. Premium brands like Authentic Urban maintain testing records and can provide reports on request.

Conclusion: Empowering Yourself to Verify Ghee Purity

Reading ghee lab test reports is a skill every informed buyer should develop. The key parameters to check are milk fat content (minimum 99%), Bauduoin Test (must be negative), moisture (under 0.5%), and RM Value (28-35 for cow ghee). These numbers tell you more about ghee quality than any marketing claim ever could.

Always verify that lab reports come from NABL accredited laboratories. Check accreditation status at nabl-india.org before trusting any certificate. When brands hesitate to share lab reports or cannot answer basic questions about their testing, treat this as a red flag. Truly transparent brands welcome scrutiny because it validates their quality investment.

For the ultimate verification, look for brands that combine lab testing with process transparency. Authentic Urban provides both: NABL lab tested purity plus video verification where you see YOUR specific jar being prepared using traditional Bilona method. This combination of scientific testing and visual proof leaves no room for doubt about what you are putting in your family's food.

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